Abstract thinking: Cardiac Arrest Research Paper
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Cardiac Arrest Research Paper Video
Cardiac Arrest: Can Education Improve Outcomes? with Dr. Lorrel BrownThis study aimed to evaluate the dysregulation of mitochondrial respiratory function after CA while testing the sampling bias that might be induced by the mitochondrial isolation method. Materials and Methods: Adult rats were subjected to min asphyxia-induced CA.
The mitochondrial quantity, expressed as protein concentration isolation yieldswas determined, and the oxygen consumption rates were measured. ADP-dependent state-3 and ADP-limited state-4 respiration activities were compared between the groups.
Background
Mitochondrial quantity was evaluated based on citrate synthase CS activity and cytochrome c Cardiac Arrest Research Paper, measured independent of the isolation yields. Conclusions: CA decreased mitochondrial respiration activity and the quantity of mitochondria isolated from the tissues. Owing to the nature of fragmented or damaged mitochondrial membranes caused by acute injury, there is a potential loss of disrupted mitochondria. Thus, it is plausible that the mitochondrial function in the acute-injury model may be underestimated as this loss is not considered. Introduction Cardiac arrest CA is a major public health issue affecting approximatelypatients each year in the United States 1. Various pathophysiological changes occur during and after CA, including mitochondrial dysfunction, multiple organ failure, and prolonged neurological dysfunction, that increase the mortality of patients with CA 2. Studies have suggested that therapies effective against mitochondrial dysfunction may improve mortality and neurological damage after CA in rodent models 34.
These results implicate the mitochondria as effectors or Cardiac Arrest Research Paper to improve the survival of patients with CA. Mitochondrial research using experimental animal models plays an important role in the study of mitochondria, and mitochondrial isolation is the foremost method for studying the pathophysiology of mitochondrial dysfunction 56.
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Therefore, the development of methods for mitochondrial purity assessment are currently in high demand, and different mitochondrial isolation methods have been reported by several investigators 78. Picard et al. They attributed the functional alterations induced by source isolation to disruption of the mitochondrial morphology caused by mechanical homogenization as well as loss of soluble proteins Cardiac Arrest Research Paper other molecules from the mitochondrial matrix. This is significant under experimental conditions given that animal models with acute injuries likely have mitochondrial disruption in nature.
However, although Picard et al. We previously reported responses to resuscitation of tissues, including the brain and kidneys, following prolonged CA by examining mitochondrial respiration using isolated mitochondria.
Brief Research Report ARTICLE
The present study aimed to evaluate mitochondrial dysfunction in the brain Carfiac kidney tissues after CA while testing the sampling bias induced by our mitochondrial isolation method, which was performed by measuring multiple indicators of the purity of mitochondrial samples. Generation of Rat CA Model and Interventions All instrumentation and surgical preparations in this non-randomized, prospective, and experimental controlled study were performed according to our previously described protocols 9.
We used male rats to avoid potential hormonal or genetic differences among animal subjects and ensure that the observed differences could be attributed to the experimental intervention i. Core temperature was maintained at The CA group included rats successfully resuscitated with cardiopulmonary resuscitation CPR after a min asphyxia.
The sham group included rats that were not treated with asphyxia or CPR. After 10 min, the animals Cardiac Arrest Research Paper resuscitated by restarting mechanical ventilation at an FIO2 of 1. The same surgical procedures were performed for rats in the sham group, including vecuronium and epinephrine injections.
Mechanical ventilation was discontinued 30 min post CPR; thereafter, the rats were euthanized, and tissues were collected for mitochondrial experiments.
All surgical procedures, including resuscitation, were performed by one investigator; therefore, blinding procedures were not performed.]
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