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Advanced Search ABSTRACT Morpholino-mediated knockdown has shown that the homeodomain transcription factors Dlx3b and Dlx4b are essential for proper induction of the otic-epibranchial progenitor domain OEPD , as well as subsequent formation of sensory hair cells in the developing zebrafish inner ear. However, increasing use of reverse genetic approaches has revealed poor correlation between morpholino-induced and mutant phenotypes. However, we find that only early-born sensory hair cells tether cells , that seed and anchor the formation of otoliths, are affected. Later-forming sensory hair cells are present, indicating that two genetically distinct pathways control the development of early-born and later-forming sensory hair cells. It derives from the otic placode, a transient ectodermal thickening adjacent to the developing hindbrain, and contains a complex arrangement of mechanosensory hair cells, nonsensory supporting cells and sensory neurons Barald and Kelley, ; Raft and Groves, ; Whitfield, Inner ear formation is a multistep process initiated by the establishment of the preplacodal region, a zone of ectoderm running around the anterior border of the neural plate containing precursors for all sensory placodes Streit, The preplacodal region is further specified into a common otic-epibranchial progenitor domain OEPD that in zebrafish also contains the progenitors of the anterior lateral line ganglion Chen and Streit, ; McCarroll et al. The otic placode develops into the otocyst or otic vesicle with mechanosensory hair cells generated in the sensory epithelia and neuronal precursors delaminating as neuroblasts from the ventral face of the otic vesicle Haddon and Lewis, ; Rubel and Fritzsch, The Road Not Taken Compare And Contrast.

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The Road Not Taken Compare And Contrast

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The Road Not Taken Compare And Contrast

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The Road Not Taken Compare And Contrast

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