Gas Chromatography Essay - what
Oil extracted by refluxing alga cells in n-hexane was used as control. Probable changes in the structures of the fatty acids were investigated using gas chromatography and Fourier transform infrared spectroscopy FT-IR. The retention profiles of the chromatograms were similar with differences observed only in peak heights. The FT-IR spectrum of the hexane extract was similar to the spectra of oil extracted at 20 and 40 kHz. The FT-IR spectra of oil extracted at 60 and 80 kHz were identical but different from the other spectra. Peaks at about cm-1 which were prominent in the 60 and 80 kHz spectra but absent from the other spectra were assigned to non-oil lipids such as steroids. It was concluded that extraction of oil from C. Key words: Alga, triglycerides, extraction, sonication, chromatography, spectroscopy. Biodiesel prepared from vegetable oils is used as fuel in compression engines. The biodiesel is used neat or blended with petrodiesel. Gas Chromatography Essay
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